[学术文献] Effects of dietaryHermetia illucensmeal inclusion on cecal microbiota and small intestinal mucin dynamics and infiltration with immune cells of weaned piglets 进入全文
Web of Science
Background The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs.Hermetia illucens(HI) meal (that represents a promising, alternative feed ingredient for production animals) has recently been demonstrated to influence colonic microbiota, bacterial metabolite profile and mucosal immune status of pigs, but no data about modulation of gut mucin dynamics are currently available. The present study evaluated the effects of dietary HI meal inclusion on the small intestinal mucin composition of piglets, as well as providing insights into the cecal microbiota and the mucosal infiltration with immune cells. Results A total of 48 weaned piglets were randomly allotted to 3 dietary treatments (control diet [C] and 5% or 10% HI meal inclusion [HI5 and HI10], with 4 replicate boxes/treatment and 4 animals/box) and slaughtered after 61 days of trial (3 animals/box, 12 piglets/diet). The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed higher beta diversity in the piglets fed the HI-based diets than the C (P < 0.001). Furthermore, the HI-fed animals showed increased abundance ofBlautia,Chlamydia,Coprococcus,Eubacterium,Prevotella,Roseburia, unclassified members of Ruminococcaceae,RuminococcusandStaphylococcuswhen compared to the C group (FDR < 0.05). The gut of the piglets fed the HI-based diets showed greater neutral mucin percentage than the C (P < 0.05), with the intestinal neutral mucins of the HI-fed animals being also higher than the sialomucins and the sulfomucins found in the gut of the C group (P < 0.05). Furthermore, the piglets fed the HI-based diets displayed lower histological scores in the jejunum than the other gut segments (ileum [HI5] or ileum and duodenum [HI10],P < 0.05). Conclusions Dietary HI meal utilization positively influenced the cecal microbiota and the small intestinal mucin dynamics of the piglets in terms of selection of potentially beneficial bacteria and preservation of mature mucin secretory architecture, without determining the development of gut inflammation. These findings further confirm the suitability of including insect meal in swine diets.
New high-protein DDGS has greater amino acid digestibility and more metabolizable energy than de-oiled DDGS but has reduced phosphorus digestibility. With a greater ability to upcycle ethanol co-products into animal feed ingredients, companies are creating custom products and partnering with University of Illinois researchers to test for quality and digestibility. In a recent study published in the Journal of Animal Science, University of Illinois researchers showed that a new high-protein dried distillers grains with solubles (DDGS) product from Marquis Energy has greater energy and protein digestibility than conventional DDGS, according to an announcement from the university.
2020年7月21日，据欧盟食品安全局（EFSA）消息，应欧盟委员会要求，欧盟动物饲料添加剂和产品（FEEDAP）研究小组就 OptiPhos PLUS（6-植酸酶（6-phytase））作为哺乳和断奶仔猪、育肥猪、母猪、其他小型育肥猪和繁殖猪饲料添加剂的安全性和有效性发表科学意见。据了解，该添加剂是一种6-植酸酶制剂，由一种转基因巴斯德毕赤酵母（Komagataella phaffii）产生。经过评估，专家小组认为该添加剂对目标物种、消费者和环境是安全的，在使用水平为250 U/kg饲料时，可能对目标物种有效。部分原文报道如下：Following a request from the European Commission, the EFSA Panel on Additives and products or Substances used in Animal Feed （FEEDAP ） was asked to deliver a scientific opinion on the safety and efficacy of OptiPhos? PLUS （6‐phytase） as a zootechnical feed additive for suckling and weaned piglets, pigs for fattening, sows, other minor pig species for fattening and other minor reproductive pig species. The additive is a preparation of 6‐phytase produced by a genetically modified strain of Komagataella phaffii and has been previously assessed by the FEEDAP Panel in the context of two applications for its use in different species/categories. The Panel concluded in those opinions that the production strain is safe, and that the use of the additive as a feed additive would raise no safety concerns for the consumers and the environment. The additive was also considered not to be skin irritant but was found to be a dermal sensitiser. The FEEDAP Panel could not conclude on the eye irritation potential of the additive. Owing to the proteinaceous nature of the active substance, the additive is considered a respiratory sensitiser. based on the tolerance studies provided, the Panel concluded that the additive is safe for the target species under the conditions of use with a wide margin of safety and therefore the conclusion were extended to suckling piglets and extrapolated to other minor pig species for fattening and to other minor reproductive pig species. The FEEDAP Panel concluded that the additive has the potential to be efficacious in weaned piglets, pigs for fattening and in sows at the level of 250 FTU /kg feed and this conclusion were extended to suckling piglets and extrapolated to other minor pig species for fattening and to other minor reproductive pig species.