[学术文献] Impact of zinc and arginine on antioxidant status of weanling piglets raised under commercial conditions 进入全文
The effects of dietary zinc and L-arginine supplements on the weight gain, feed efficiency, antioxidant capacity and oxidative status of weanling piglets raised under commercial conditions were examined. A total of 288 piglets aged 21 d were fed for 15 d a diet supplemented or not with 2,500 mg/kg of zinc (provided as zinc oxide) and 1% L-arginine·HCl. The 4 treatments were distributed in a randomized complete block design with 6 initial body weight categories (12 animals per pen). Access to feed and water was ad libitum . Data were analyzed as a 2 × 2 factorial experiment using the SAS MIXED procedure, with zinc and arginine as the main independent variables. Blood collection day (d 8 and 15, samples were collected from the same 2 piglets in each pen before the morning feeding) was included as a third factor. The zinc supplement increased the average daily gain (ADG) from d 0 to 7, d 8 to 15 and d 0 to 15 (0.289 vs. 0.217 kg/d), average daily feed intake (ADFI) from d 8 to 15 and d 0 to 15 (0.338 vs. 0.279 kg/d) and the gain to feed (G:F) ratio from d 0 to 7 and d 0 to 15 (0.86 vs. 0.77) ( P < 0.001). Both supplements significantly decreased the malondialdehyde concentration (zinc: 4.37 vs. 3.91 μmol/L, P = 0.005; arginine: 4.38 vs. 3.89 μmol/L, P = 0.002). Total antioxidant capacity and reduced glutathione (GSH) increased from d 8 to 15 (0.953 vs. 1.391 μmol/L, 2.22 vs. 3.37 μmol/L, P < 0.05) regardless of dietary treatment. Total and oxidized GSH concentrations on d 8 were higher in response to the combined supplements (zinc × arginine interaction, P < 0.05). Piglets fed either Zn-supplemented diet had a lower haptoglobin serum concentration (509 vs. 1,417 mg/L; P < 0.001). In conclusion, the zinc supplement improved piglet growth performance (ADG and ADFI) and oxidative status (based on malondialdehyde concentration). The arginine supplement had a limited effect on growth performance and oxidative status under these conditions.
[学术文献] Dietary apple polyphenols supplementation enhances antioxidant capacity and improves lipid metabolism in weaned piglets 进入全文
Apple polyphenols (APPs) are biologically active flavonoids that have antioxidant, anti‐inflammatory, improving insulin sensitivity, hypocholesterolaemic effect and antiviral properties. This study was conducted to explore effects of dietary APPs supplementation on antioxidant activities and lipid metabolism in weaned piglets. Fifty‐four weaned piglets (half male and female) were randomly divided into three groups with six replicates in each group and three piglets in each repetition. Piglets were fed control diet (basal diet) or a control diet supplemented with 400 mg/kg or 800 mg/kg APPs for 6 weeks. Blood and liver samples were collected to determine biochemical, antioxidant and lipid metabolism parameters. Here we showed that dietary APPs supplementation increased HDL‐C and decreased T‐CHO, TG and LDL‐C concentrations. Dietary APPs supplementation increased antioxidative capacity in serum and CAT activity in liver, and significantly increased the mRNA expressions of CAT , GST and SOD1 in liver. ACC mRNA level and LPL activity were tended to decrease by APPs. HMG‐CoAR , CTP7A1 , CD36 and FATP1 mRNA levels were decreased by APPs, while LDL‐R , PGC‐1α , Sirt1 and CPT1b mRNA levels were increased by 400 mg/kg APPs. No alterations in growth performance were found in all treatments. This study firstly provided the evidence that dietary APPs supplementation could enhance systemic antioxidant capacity and improve lipid metabolism in weaned piglets. The mechanism by which APPs improve lipid metabolism might be through regulating hepatic cholesterol metabolism and increasing fatty acid oxidation, and decreasing fatty acid uptake and de novo synthesis.
猪传染性胃肠炎病毒(transmissible gastroenteritis virus,TGEV)感染仔猪能够引起仔猪肠道损伤,导致仔猪腹泻脱水甚至死亡,其致病机制主要在于破坏猪小肠黏膜,引起肠道黏膜上皮屏障功能受损,具体主要表现为:TGEV破坏仔猪肠道上皮机械屏障完整和电解质平衡,扰乱生物菌群结构,降低局部免疫功能。TGEV诱导炎症及先天免疫反应的作用机制主要通过激活维甲酸诱导基因Ⅰ样受体(RLR)和Toll样受体(TLR),使核转录因子(NF-κB)活化,进一步诱导细胞因子和干扰素表达。通过饲粮中添加氨基酸、维生素和中草药等营养调控措施可以达到缓解TGEV损伤肠道屏障功能的目的,为预防及治疗TGEV感染对仔猪肠道屏障功能的影响提供参考。