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Gold nanoparticles-based multifunctional nanoconjugates for highly sensitive and enzyme-free detection of E.coli K12

金纳米粒子为基础的多功能纳米偶联物,用于大肠杆菌K12的高灵敏度和无酶检测

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来源:
TALANTA
全文链接1:
file1/M00/06/5D/Csgk0FxRVsyAEiQlABwWrH2FcmM341.pdf
全文链接2:
类型:
学术文献
语种:
英文
原文发布日期:
2018-09-19
摘要:
Immobilization of proteins on a biocompatible conductive interface is highly desirable for the fabrication of biosensors. In this study, a nanocomposite has been prepared by assembling well-distributed gold nanoparticles (AuNPs) on the surface of a polypyrrole-reduced graphene oxide (PPy-rGO) composite through electrostatic adsorption. This serves as a platform for immobilization of a capture antibody, which was conjugated onto the ferrocene doped polypyrrole-gold nanoparticles (PPy@Fc/AuNPs) composite. The design and performance of the biosensor was tested against detection of a whole-cell bacteria E. coli K12. This nanocomposite has a high surface area, good conductivity and biocompatibility, which is shown to be very suitable for enzyme-free detection of this bacteria. Results show excellent analytical performance with a linear range from 1.0 x 10(1) to 1.0 x 10(7) CFU mL(-1) and a low detection limit of 10 CFU mL(-1). The sensor has high selectivity, excellent reproducibility, and good stability.
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