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Transcriptional Profiling Reveals a Time-of-day Specific Role of REVEILLE 4/8 in Regulating the First Wave of Heat Shock-induced Gene Expression in Arabidopsis

热激以不同生物钟的调节机制来调节RVE4/8的转录活性进而调节热激响应基因的表达

关键词:
来源:
THE PLANT CELL
全文链接1:
http://agri.ckcest.cn/topic/downloadFile/4422129a-c5e5-442f-93bb-41be31ce51a1
全文链接2:
http://www.plantcell.org/content/early/2019/07/29/tpc.19.00519
类型:
学术文献
语种:
英语
原文发布日期:
2019-07-29
摘要:
Although much is known about plant responses to heat shock (HS), how plants sense high temperature and the primary HS signal transduction pathway leading to HS-regulated gene expression are still poorly understood. To identify primary transcription factors that mediate HS-regulated gene expression and their target genes, RNA-seq was performed to detect genes whose expression is rapidly altered by HS in Arabidopsis thaliana. The results showed several genes were induced after only 5 min of HS treatment, suggesting that HS signaling occurs very rapidly. Analysis of the cis-elements in the promoters of genes upregulated by 10 min of HS treatment identified HEAT SHOCK FACTOR A1s (HSFA1s) and circadian clock proteins REVEILLE4/8 (RVE4/8) as essential transcription factors that independently mediate early HS-induced gene expression. Using hsfa1a/b/d/e and rve4/8 mutants, we identified subsets of HSFA1s- or RVE4/8-dependent early HS-induced genes, and showed RVE4/8 regulate plant thermotolerance partially by regulating the expression of downstream transcription factors ETHYLENE RESPONSIVE FACTOR53 (ERF53) and ERF54, specifically around noon. These findings reveal a potential transcriptional regulatory hierarchy governing the first wave of HS-induced gene expression. They also provided important insight into the mechanism by which the circadian clock gates thermotolerance and prepares plants for exposure to high temperatures during the day.
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