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Microfluidic based impedance biosensor for pathogens detection in food products

基于微流体的阻抗生物传感器用于食品中病原体的检测

关键词:
来源:
ELECTROPHORESIS
全文链接1:
file1/M00/06/69/Csgk0FytRgmAbvUgACAR2qlLF5Y226.pdf
全文链接2:
类型:
学术文献
语种:
英文
原文发布日期:
2018-12-16
摘要:
A MEMS-based impedance biosensor was designed, fabricated, and tested to effectively detect the presence of bacterial cells including E. coli O157:H7 and Salmonella typhimurium in raw chicken products using detection region made of multiple interdigitated electrode arrays. A positive dielectrophoresis based focusing electrode was used in order to focus and concentrate the bacterial cells at the centerline of the fluidic microchannel and direct them toward the detection microchannel. The biosensor was fabricated using surface micromachining technology on a glass substrate. The results demonstrate that the device can detect Salmonella with concentrations as low as 10 cells/mL in less than 1 h. The device sensitivity was improved by the addition of the focusing electrodes, which increased the signal response by a factor between 6 and 18 times higher than without the use of the focusing electrodes. The biosensor is selective and can detect other types of pathogen by changing the type of the antibody immobilized on the detection electrodes. The device was able to differentiate live from dead bacteria.
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