Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods

负责人：

关键词：

Phyllocnistis citrella;PCR cycles;Orius majusculus;Entomobrya sp.;PCR bias;Theridion sp.;annealing temperature;Macrolophus melanotoma;Orius laevigatus;Philodromus cespitum;Adalia decempunctata;Trichopsocus clarus;insects;Episyrphus balteatus;Ion Torrent;Aphidius colemani;Aphis spiraecola

摘要：

ble annealing temperature and number of PCR cycles. The results show that reads of all species were recovered after PCR enrichment at our control conditions (no bloc