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Data from: Fast and cost-effective genetic mapping in apple using next-generation sequencing
负责人:
关键词:
Malus x domestica
DOI:
doi:10.5061/dryad.55t54
摘要:
Next-generation DNA sequencing (NGS) produces vast amounts of DNA sequence data, but it is not specifically designed to generate data suitable
Data from: Unforeseen consequences of excluding missing data from next-generation sequences: simulation study of RAD sequences
负责人:
关键词:
RADtags;species delimitation;phylogenetic;Next-generation sequencing;RADseq;phylogeography
DOI:
doi:10.5061/dryad.jf361
摘要:
simulations, focusing specifically on RAD sequences, to highlight some of the unforeseen consequence of excluding missing data from next-generation sequencing
Data from: CHOPER filters enable rare mutation detection in complex mutagenesis populations by next-generation sequencing
负责人:
关键词:
NGS;bioinformatics;Next Generation Sequencing
DOI:
doi:10.5061/dryad.mv827
摘要:
Next-generation sequencing (NGS) has revolutionized genetics and enabled the accurate identification of many genetic variants across many ge
Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows
负责人:
关键词:
Amplicon Sequencing;high throughput sequencing;DNA metabarcoding;Second generation sequencing;Next-generation sequencing
DOI:
doi:10.5061/dryad.2qf0t
摘要:
Amplicon sequencing has been the method of choice in many high-throughput DNA sequencing (HTS) applications. To date there has been a heavy focus
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
负责人:
Brelsford, Alan
关键词:
Population genomics AFLP 454 PCR proofreading polymerase high-fidelity polymerase
DOI:
doi:10.5061/dryad.1hg75
摘要:
High-fidelity “proofreading” polymerases are often used in library construction for next-generation sequencing projects, in an effort to minimize
Data from: A first look at the Oxford Nanopore MinION sequencer
负责人:
关键词:
Genomics\/Proteomics;nanopore sequencing;Next-generation sequencing;bioinformatics;Protobothrops flavoviridis
DOI:
doi:10.5061/dryad.5p0c3
摘要:
next-generation sequencing platforms. The MinION is an exciting step in a new direction for single-molecule sequencing, though it will require dramatic decrease
Data from: Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies
负责人:
关键词:
454;Ion Torrent;Clostridium autoethanogenum;Sanger;Methylation;Illumina;PacBio;Next Generation DNA Sequencing
DOI:
doi:10.5061/dryad.6fm1p
摘要:
During the past decade, DNA sequencing output has been mostly dominated by the second generation sequencing platforms which are character
Data from: The QRS (Quantification of Representative Sequences) pipeline for amplicon sequencing: case study on within-population ITS1
负责人:
关键词:
cloning\/sanger sequencing;ITS1 region;Caullerya mesnili;Amplicon Sequencing
DOI:
doi:10.5061/dryad.v7f7r
摘要:
Next generation sequencing (NGS) platforms are replacing traditional molecular biology protocols like cloning and Sanger sequencing. However
Data from: Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison
负责人:
关键词:
Formalin-fixed paraffin-embedded;Colorectal cancer;Targeted next-generation sequencing;RAS mutations;Ion Torrent Personal Genome Machine
DOI:
doi:10.5061/dryad.3447g
摘要:
Objective: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations detecti

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