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Data from: The effects of read length, quality and quantity on microsatellite discovery and primer development: from Illumina to PacBio
- 负责人：
- 摘要：
- luding single-molecule real-time DNA sequencing (Pacific Biosciences, or PacBio) remain viable alternatives. We outline a workflow from sequence quality control
Data from: Small RNAs from a big genome: the piRNA pathway and transposable elements in the salamander species Desmognathus fuscus
- 负责人：
- Mueller, Rachel L.
- 关键词：
- genome size evolution small RNA evolution transposable element silencing piRNA pathway salamanders vertebrates
- 摘要：
- of the ancestral piRNA-mediated TE-silencing machinery. We characterized the small RNA pool in the female and male adult gonads, testing for the presenc
Data from: High-throughput sequencing of nematode communities from total soil DNA extractions
- 负责人：
- 摘要：
- alternative. Generally, primers that have been used for generating amplicons for sequencing are not nematode specific and also amplify other groups suc
Data from: A new plant virus discovered by immunocapture of double stranded RNA; assessment of a novel approach for viral metagenomics studies
- 负责人：
- Blouin, Arnaud G.
- 关键词：
- Metagenomics antibodies dsRNA NGS virus
- 摘要：
- decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. As the majority
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
- 负责人：
- Brelsford, Alan
- 摘要：
- nt for sequencing applications, we suggest that it may be more effective to use alternative library preparation methods.
Data from: Ion Torrent PGM as tool for fungal community analysis: a case study of endophytes in Eucalyptus grandis reveals high taxonomic diversity
- 负责人：
- 摘要：
- . This is especially true for endophytic communities of fungi living in healthy plant tissue. The developments in next generation sequencing technologies ar
Data from: Less is more: extreme genome complexity reduction with ddRAD using Ion Torrent semiconductor technology
- 负责人：
- 关键词：
- Eurasian perch SNP development and validation de novo assembly next-generation sequencing population genomics DNA pooling
- 摘要：
- rasian perch. We expect that our ddRAD sequencing protocol for semiconductor sequencing technology will be useful alternative for currently available RAD protocols.
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
- 负责人：
- 摘要：
- s. Molecular analyses of these gene families require high-confidence sequences, but the high sequence similarity of the members can create challenges for sequencing
Data from: On the optimal trimming of high-throughput mRNA sequence data
- 负责人：
- 摘要：
- to data quality is required. In particular, because high-throughput sequencing is more error-prone than traditional Sanger sequencing, quality trimming of sequence
Data from: Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns
- 负责人：
- Bunce, Michael
- 摘要：
- . Here we show that Second Generation, high-throughput sequencing (HTS) of DNA represents an effective means to genetically audit organic ingredients
