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Data from: Small RNAs from a big genome: the piRNA pathway and transposable elements in the salamander species Desmognathus fuscus
负责人:
Mueller, Rachel L.
关键词:
genome size evolution small RNA evolution transposable element silencing piRNA pathway salamanders vertebrates
摘要:
of the ancestral piRNA-mediated TE-silencing machinery. We characterized the small RNA pool in the female and male adult gonads, testing for the presenc
Data from: High-throughput sequencing of nematode communities from total soil DNA extractions
负责人:
关键词:
Agriculture;nematode;soil;Nematoda
摘要:
alternative. Generally, primers that have been used for generating amplicons for sequencing are not nematode specific and also amplify other groups suc
Data from: A new plant virus discovered by immunocapture of double stranded RNA; assessment of a novel approach for viral metagenomics studies
负责人:
Blouin, Arnaud G.
关键词:
Metagenomics antibodies dsRNA NGS virus
摘要:
decrease in sequencing costs, it is difficult to justify their use in large-scale surveys without a virus sequence enrichment technique. As the majority
Data from: Nonspecific PCR amplification by high-fidelity polymerases: implications for next-generation sequencing of AFLP markers.
负责人:
Brelsford, Alan
关键词:
Population genomics AFLP 454 PCR proofreading polymerase high-fidelity polymerase
摘要:
nt for sequencing applications, we suggest that it may be more effective to use alternative library preparation methods.
Data from: Ion Torrent PGM as tool for fungal community analysis: a case study of endophytes in Eucalyptus grandis reveals high taxonomic diversity
负责人:
关键词:
Fungi;Eucalyptus grandis;metagenomics;internal transcribed spacer (ITS);Endophytes
摘要:
. This is especially true for endophytic communities of fungi living in healthy plant tissue. The developments in next generation sequencing technologies ar
Data from: Less is more: extreme genome complexity reduction with ddRAD using Ion Torrent semiconductor technology
负责人:
关键词:
Eurasian perch SNP development and validation de novo assembly next-generation sequencing population genomics DNA pooling
摘要:
rasian perch. We expect that our ddRAD sequencing protocol for semiconductor sequencing technology will be useful alternative for currently available RAD protocols.
Data from: Evaluating the performance of targeted sequence capture, RNA-Seq, and degenerate-primer PCR cloning for sequencing the largest mammalian
负责人:
关键词:
Genomics\/Proteomics;targeted sequence capture;Transcriptomics;Mammals;olfactory receptor;multigene family
摘要:
s. Molecular analyses of these gene families require high-confidence sequences, but the high sequence similarity of the members can create challenges for sequencing
Data from: On the optimal trimming of high-throughput mRNA sequence data
负责人:
关键词:
Trinity;genomics;RNAseq
摘要:
to data quality is required. In particular, because high-throughput sequencing is more error-prone than traditional Sanger sequencing, quality trimming of sequence
Data from: Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns
负责人:
Bunce, Michael
关键词:
Traditional Chinese Medicine
摘要:
. Here we show that Second Generation, high-throughput sequencing (HTS) of DNA represents an effective means to genetically audit organic ingredients

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