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Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus
负责人:
关键词:
metagenomics;Buchnera aphidicola;Wolbachia;Cinara;Arthropod;Aphididae;endosymbiont;Illumina;Lachninae;Serratia;16S rRNA gene;Enterobacteriaceae
摘要:
e therefore crucial in arthropod microbiome studies. We aimed here to validate an Illumina 16S rRNA gene sequencing protocol and analytical pipeline
Data from: Comparative study of the validity of three regions of 18S-rRNA gene for massively parallel sequencing-based monitoring of the planktonic
负责人:
关键词:
Environmental DNA;metagenomics;marine eukaryote;Metabarcoding;Bioinfomatics\/Phyloinfomatics;Community Ecology
摘要:
The nuclear 18S-rRNA gene has been used as a metabarcoding marker in massively parallel sequencing (MPS)-based environmental surveys for plankton
Data from: Deep sequencing of a candidate region harboring the SOX9 gene for the canine XX disorder of sex development
负责人:
Grzemski, Adrian
关键词:
dog intersexuality DSD SOX9 MAGI2 CNV lincRNA RevSex
摘要:
and two blocks of an elevated heterozygosity. An initial next-generation sequencing analysis showed an association with two SNPs, but validation in larger cohorts
Data from: Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly
负责人:
关键词:
Genomics\/Proteomics;Parasteatoda tepidariorum;molecular evolution;Bioinfomatics\/Phyloinfomatics;Invertebrates;spider
摘要:
e obtained. The parameters for sample storage, RNA extraction, library preparation, sequencing, and in silico assembly considered in this work provide
Data from: Metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples
负责人:
关键词:
Metazoan;Midori reference
摘要:
Mitochondrial-encoded genes are increasingly targeted in studies using high-throughput sequencing approaches for characterizing metazoan communities
Data from: High-throughput amplicon sequencing of rRNA genes requires a copy number correction to accurately reflect the effect
负责人:
关键词:
burning;metagenomics;Nitrogen enrichment;Konza prairie;tallgrass prairie;nematodes
摘要:
frequencies based on amplicon sequencing were not initially consistent with specimen-based counts, but correction for differences in rRNA gene copy number usi
Data from: Don’t put all your eggs in one basket: a cost-effective and powerful method to optimize primer choice for rRNA environmental community
负责人:
关键词:
Fungi;Fluidigm;Mock Community;ITS;Primer Selection;LSU
摘要:
With the increasing democratization of high-throughout sequencing (HTS) technologies, along with the concomitant increase in sequence yield pe
Data from: Efficient detection of novel nuclear markers for Brassicaceae by transcriptome sequencing
负责人:
关键词:
Primer-design;Arabidopsis thaliana;primer database;Next-generation sequencing;RNA-seq;phylogenetics;Cardamine hirsuta;Arabis alpina;454 transcriptome sequencing;Brassicaceae
摘要:
of molecular polymorphisms in nuclear markers. Next-generation sequencing (NGS) techniques nowadays provide a powerful approach to overcome this limitation. We present
Data from: Immunomics of the koala (Phascolarctos cinereus )
负责人:
关键词:
annotation;koala retrovirus;spleen;vertebrate;mRNA;Major histocompatibility complex;ribosomal RNA;San Diego Zoo koala colony;RIG-I like receptor;buffy coat;cDNA;Phascolarctos cinereus;Toll-like receptor
摘要:
antigens—and to help combat infectious diseases (e.g., chlamydiosis) that impede ongoing conservation efforts. We used Illumina sequencing of cDNA
Data from: Discovery and evaluation of candidate sex-determining genes and xenobiotics in the gonads of lake sturgeon (Acipenser fulvescens)
负责人:
关键词:
Acipenser fulvescens;polyploidy;Trichomonas;transcriptome;Pyrosequencing 454;Schistosoma
摘要:
(Acipenser fulvescens). Using 454 pyrosequencing of transcribed genes, we produced more than 125 MB of sequence data from 473,577 high-quality sequencing reads. Sequences

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